Occurrence of caffeine in carbonated drinks cola type from Romanian market

نویسنده

  • MIRCEA OROIAN
چکیده

Caffeine is a natural substance present in leaves and seeds of many plants. The aim of this study is to evaluate the caffeine level in the carbonated drinks from the Romanian market. For this survey were taken 30 samples of different carbonated drinks. The caffeine was determined and quantified using a HPLC with diode array detector at 206 nm. The caffeine retention time was at 17.785 minutes. The calibration curve was build using 3 stock solution of caffeine (25, 50 and 100 mg/L). The equation of the calibration curve was y=176460x, with a correlation coefficient R=0.999. The caffeine content in carbonated drinks from the Romanian market ranged between 25.20 – 109.68 mg/L. Key-Words: caffeine, carbonated drinks, HPLC, UV detection, risk assessment, hazard quotient

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تاریخ انتشار 2013